0. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. SpectraMax Multi-Mode Microplate Readers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. MMP‑1 inhibition assay. 7 macrophages were obtained from ATCC (Manassas, VA). reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). 0 3. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. In this study we used tetanus toxoid (TT) as a well-defined antigen to. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. The contents of the. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Triton X-100 (see Note 2). Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. 5 mL of 1 M acetate buffer (pH 4. 4. 2. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Lactate Dehydrogenase (LDH) Release. View the Molecular Devices website. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. A. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Microplate Readers. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). 81. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Warranty. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. Briefly, cells were seeded into a. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). Features showcased by this model include. SpectraMax Multi-Mode Microplate Readers. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. ATI/240, 250. , Sunnyvale, CA). Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax Multi-Mode Microplate Readers. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Detection limits. Donor filter: 616/10 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. Bioz Stars score: 86/100, based on 1 PubMed citations. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. 5 mL of 1 M acetate buffer (pH 4. 5. Amino Acid Analysis. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. TDP-43 stress granular cells were treated with 70% ice-cold. Data were expressed as the mean LPS-treated cells (100%) vs. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 0200-6117. 4) was followed according to Vanacker et al. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. SpectraMAX 340 microtiter plate reader. , Sunnyvale CA). Labsystems Luminoskan RT Microplate Reader. Briefly, cells were sequentially treated with terminal deoxynucleotidyl. Please see photos. Molecular Devices SpectraMax M5e Microplate Reader. TGFα release was calculated as described in the Results. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Wavelength Range. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Page: 1/6. Acceptor: 665nm. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 9000-0756. Wells were washed three times. The Catalina. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 5 ng/ml; estradiol: 10 pg/ml. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Microplate Readers. 5 mL sample or standard solution was well mixed with 0. The amount of liberated pNA product during incubations was determined using a standard curve obtained at 410 nm for various concentrations of a pNA stock solution in the SpectraMax 340 microplate reader. SoftMax Pro. Microplate Spectrophotometer Operator’s Manual. The SpectraMax® iD3 Multi Mode Microplate . more on but is otherwise untested and sold AS-IS. Intra- and inter-assay coefficients of variation were lower than 8. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. 313 reference gain check fail. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. Wound healing assay. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . 3: The cuvette chamber. Molecular Devices Spectramax 340PC instructions. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. Acceptor: 665/10 nm. Briefly, cells were seeded into a. 5 mL sample or standard solution was well mixed with 0. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. Awareness Technology Inc. Cell culture 3T3 fibroblasts and RAW 264. 340 nm. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. SpectraMax Multi-Mode Microplate Readers. Binding of capture antibodies to polystyrene beads and biotinylation. 30. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Donor: 620 nm. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. using SpectraMax i3 (Molecular devices, San Jose, USA). SpectraMax 190 reads 96-well and strip-well plates. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 00. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . The VersaMax and SpectraMax 190 read 96-well microplates. SpectraMax Plus 384 measuring instruments pdf manual download. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Wavelength Selection. Amino Acid Analysis. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. edu Contact Phone: 216-368-8962. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax ® Plus 384 optics. Oxidative stress induction by H 2 O. 4. 0% and ± 0. Stat Fax 3200 Microplate Reader. Anti-proteolytic activity. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The ABS reader ranges from. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. Dissolve 13. The area under the curve (AUC) for the comparison of the growth rates was determined using. SPECTRAmax® 190. (1993). The plate was covered and incubated for one hour at room temperature. g. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. chen@case. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Call 1-800-745-2710. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Call 1-800-745-2710. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. Novak, Nathan J. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Cet appareil ISM est confomre à la norme NMB-001 du Canada. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). control. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 340/70 nm. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Create custom fluorescence assays without having to purchase expensive filter sets. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. If depot service is the best option for you we will assist you with logistics to help you keep. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). #N207) LAL Reagent Water (Lonza cat. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. 024). The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Subsequently, the mixture was heated at 100 °C for 10. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. This assay measures the two major sugars in wine, glucose and fructose. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Pharmacokinetic and pharmacodynamic analysis. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. One plate carrier adapter is provided with the instrument. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 313 reference gain check fail. Microscopy. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. 6. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. . When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. PerkinElmer. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. 312 gain calibration failed. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). 5% phosphoric acid) in microplates at room temperature for 10 min. Call 1-800-745-2710. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Money Saved: $340. Six wells were assayed for each set of conditions and stadard deviations. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. SpectraMax 190 reads 96-well and strip-well plates. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Homeostatic model assessment for insulin. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. TDP-43 stress granular cells were treated with 70% ice-cold. One plate carrier adapter is provided with the instrument. P. Subsequently, the mixture was heated at 100 °C for 10. Each SpectraMax® 340 PC 384 Microplate Reader has an. 2. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 5 g/L-EDTA 0. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. 1% PEG 8000 at 37°C. Details. 3 0. Manager: Yinghua Chen, PhD Contact Email: yinghua. 5. Immunofluorescence and Aggregation Analysis. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 570nm on the indicated days (Cell Proliferation Kit I; Roche). Summary of Contents for Molecular Devices SPECTRAmax 340PC. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Plates were washed with 1× KP solution and blocked with. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). Molecular Devices SpectraMax M5e Microplate Reader $26,500. Bertin Precellys Evolution Touch Homogenizer. Or scan up to 384 wells at any time to determine the best assay conditions. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. ). 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Analysis of ALA by Ehrlich’s Reagent Assay. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 5mM), and changes in OD 405 nm. Description 1. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Unit powers. gp70 blocking. Molecular Device M2e. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. As a common indicator of equine T cell activation, cytokine induction. 2. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Exhibit with us {{>currencyLabel}} Back. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. StakMax Microplate Handling Stacker. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. #ABS PLUS) Methods. To correct for inter. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). 30. The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 340 nm / 80 nm. 304 signal level saturation. using SpectraMax i3 (Molecular devices, San Jose, USA). Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. The values were corrected for nonspecific background by subtracting the OD 650. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. Number of flashes. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. The reaction mixture was incubated for 5 min. Subsequent replacements require only the lamp (N-34-LB) be replaced. 190–850 nm <± 1. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 1 0 100 80 60 40 20 0 4. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . Lactate Dehydrogenase (LDH) Release. The light features a slim profile to prevent. , Sunnyvale, Calif. 5 - 2. Stat Fax 4700 Microstrip Reader. Isolation of RNA and RT-PCR. Gerritsma,B. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Cell proliferation was measured using the MTT method. Molecular Devices SpectraMax Gemini XS Microplate Reader. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). 5% phosphoric acid) in microplates at room temperature for 10 min. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. Example: [Drawer]. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 2 M DEEMM were mixed in a microtube,. using SpectraMax i3 (Molecular devices, San Jose, USA). SpectraMax Injector Cartridge with SmartInject™ Technology. D2. reader (Molecular Devi ces). Call 1-800-745-2710. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. 3 mm. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). 304 signal level saturation. Quick View. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. Water – distilled water or equivalent. Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4.